unglycosylated rhifn α2 (Miltenyi Biotec)
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unglycosylated rhifn α2
Unglycosylated Rhifn α2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/Human+IFN-%CE%B12a%2C+research+grade/pmc12984024-278-41-46
Average 95 stars, based on 63 article reviews
Unglycosylated Rhifn α2, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ifn+%CE%B12/Human+IFN-%CE%B12a%2C+research+grade/pmc12984024-278-41-46
Average 95 stars, based on 63 article reviews
unglycosylated rhifn α2 - by Bioz Stars,
2026-10
95/100 stars
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Transfection:Article Title: Impaired serum neutralization and death in Omicron-infected critically ill patients: insights from the French SEVARVIR prospective, multicenter cohort study. Article Snippet: .. Cells were transfected in the presence of the X-tremeGene9 transfection reagent (Sigma-Aldrich, ref. number 6365779001) for 24 h. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum (after inactivation at 56 °C, for 20 min) were either left unstimulated or were stimulated with Article Title: Impaired serum neutralization and death in Omicron-infected critically ill patients: insights from the French SEVARVIR prospective, multicenter cohort study Article Snippet: .. Cells were transfected in the presence of the X-tremeGene9 transfection reagent (Sigma-Aldrich, ref. number 6365779001) for 24 h. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum (after inactivation at 56 °C, for 20 min) were either left unstimulated or were stimulated with Article Title: Incontinentia pigmenti underlies thymic dysplasia, autoantibodies to type I IFNs, and viral diseases. Article Snippet: Briefly, HEK293T cells were transfected with a pGL4.45 plasmid containing the firefly luciferase genewith five ISRE (59-GGGAAAGTGAAACTA-39) in the promoter and a pRL-SV40 plasmid constitutively expressing the Renilla luciferase for normalization (#E2231; Promega). .. Cells were transfected in the presence of the X-tremeGene 9 transfection reagent (#6365779001; Roche) for 16 h. Cells in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum/plasma were either left unstimulated orwere stimulated with Modification:Article Title: Impaired serum neutralization and death in Omicron-infected critically ill patients: insights from the French SEVARVIR prospective, multicenter cohort study. Article Snippet: .. Cells were transfected in the presence of the X-tremeGene9 transfection reagent (Sigma-Aldrich, ref. number 6365779001) for 24 h. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum (after inactivation at 56 °C, for 20 min) were either left unstimulated or were stimulated with Article Title: Impaired serum neutralization and death in Omicron-infected critically ill patients: insights from the French SEVARVIR prospective, multicenter cohort study Article Snippet: .. Cells were transfected in the presence of the X-tremeGene9 transfection reagent (Sigma-Aldrich, ref. number 6365779001) for 24 h. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum (after inactivation at 56 °C, for 20 min) were either left unstimulated or were stimulated with Article Title: Compound heterozygous mutations in the kinase domain of IKKα lead to immunodeficiency and immune dysregulation Article Snippet: Cells were transfected in the presence of the X-tremeGene 9 transfection reagent (Sigma Aldrich, ref. number 6365779001) for 24 hours. .. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum/plasma were either left unstimulated or were stimulated with Article Title: Incontinentia pigmenti underlies thymic dysplasia, autoantibodies to type I IFNs, and viral diseases. Article Snippet: Briefly, HEK293T cells were transfected with a pGL4.45 plasmid containing the firefly luciferase genewith five ISRE (59-GGGAAAGTGAAACTA-39) in the promoter and a pRL-SV40 plasmid constitutively expressing the Renilla luciferase for normalization (#E2231; Promega). .. Cells were transfected in the presence of the X-tremeGene 9 transfection reagent (#6365779001; Roche) for 16 h. Cells in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum/plasma were either left unstimulated orwere stimulated with Control:Article Title: Impaired serum neutralization and death in Omicron-infected critically ill patients: insights from the French SEVARVIR prospective, multicenter cohort study. Article Snippet: .. Cells were transfected in the presence of the X-tremeGene9 transfection reagent (Sigma-Aldrich, ref. number 6365779001) for 24 h. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum (after inactivation at 56 °C, for 20 min) were either left unstimulated or were stimulated with Article Title: Impaired serum neutralization and death in Omicron-infected critically ill patients: insights from the French SEVARVIR prospective, multicenter cohort study Article Snippet: .. Cells were transfected in the presence of the X-tremeGene9 transfection reagent (Sigma-Aldrich, ref. number 6365779001) for 24 h. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum (after inactivation at 56 °C, for 20 min) were either left unstimulated or were stimulated with Article Title: Compound heterozygous mutations in the kinase domain of IKKα lead to immunodeficiency and immune dysregulation Article Snippet: Cells were transfected in the presence of the X-tremeGene 9 transfection reagent (Sigma Aldrich, ref. number 6365779001) for 24 hours. .. Cells in Dulbecco’s modified Eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum/plasma were either left unstimulated or were stimulated with Article Title: Post-transfusion activation of coagulation pathways during severe COVID-19 correlates with COVID-19 convalescent plasma antibody profiles Article Snippet: Cells were transfected with the X-tremeGene9 transfection reagent (Sigma-Aldrich, 6365779001) for 24 hours. .. Cells in DMEM (Thermo Fisher Scientific) supplemented with 2% fetal calf serum and 10% healthy control or patient serum (after inactivation at 56°C for 20 minutes) were either left unstimulated or stimulated with Article Title: Incontinentia pigmenti underlies thymic dysplasia, autoantibodies to type I IFNs, and viral diseases. Article Snippet: Briefly, HEK293T cells were transfected with a pGL4.45 plasmid containing the firefly luciferase genewith five ISRE (59-GGGAAAGTGAAACTA-39) in the promoter and a pRL-SV40 plasmid constitutively expressing the Renilla luciferase for normalization (#E2231; Promega). .. Cells were transfected in the presence of the X-tremeGene 9 transfection reagent (#6365779001; Roche) for 16 h. Cells in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 2% fetal calf serum (FCS) and 10% healthy control or patient serum/plasma were either left unstimulated orwere stimulated with Article Title: Prevalence and association of type I IFN autoantibodies with clinical outcomes in critically ill Brazilian COVID-19 patients Article Snippet: Cells were transfected in the presence of Lipofectamine 3000TM transfection reagent (reference number L3000015; Invitrogen) for 16 hours. .. Cells in DMEM (Thermo Fisher Scientific) supplemented with 2% FCS and 10% healthy control or patient plasma (after inactivation at 56 °C, for 20 minutes) were either left unstimulated or were stimulated with Concentration Assay:Article Title: Case-based evidence links increased SARS-CoV-2 diversity to impaired IFN-I response in severe COVID-19 Article Snippet: .. Subsequently, sera diluted 1/10 in duplicate were added to Clinical Proteomics:Article Title: Prevalence and association of type I IFN autoantibodies with clinical outcomes in critically ill Brazilian COVID-19 patients Article Snippet: Cells were transfected in the presence of Lipofectamine 3000TM transfection reagent (reference number L3000015; Invitrogen) for 16 hours. .. Cells in DMEM (Thermo Fisher Scientific) supplemented with 2% FCS and 10% healthy control or patient plasma (after inactivation at 56 °C, for 20 minutes) were either left unstimulated or were stimulated with |
